National Reference Laboratory for Campylobacteriosis
in Animals

1. NRL experts

• NRL coordinator

  Dr. Romaşcu Luminiţa, master in genetics, microbiology and biotechnology
  tel. 0374322007
  e–mail: luminita.romascu@idah.ro

• NRL deputy coordinator

  Dr. Vlaicu Viorica, post-university studies in veterinary laboratory diagnosis

• Team working

  Dr. Ionescu Maria, PhD candidate
  Dr. Iordache Lucia, PhD in medical sciences, veterinary medicine specialty
  Dr. Pricop Lavinia, PhD candidate
  Dr. Brad Gratziela
  Dr. Stoichici Anton

2. Correspondent Community Reference Laboratory

• Community Reference Laboratory for Campylobacter, National Veterinary Institute, SVA, SE-751 89 Uppsala, Sweden

3. Specific atributions

• The specialists working in this NRL have the responsibility for drawing up the handbook: „Manual for Diagnosis of Campylobacteriosis in Animals”.
• They also perform the changes and/or supplements for „Manual for Diagnosis of Campylobacteriosis in Animals” in accordance with new provisions of Community law and new recommendations of the World Animal Health Organization, established in „Manual of Diagnostic Tests and Vaccines for Terrestrial Animals” and „Terrestrial Animal Health Code”.
• NRL experts approve the results of validity studies proving the reliability of methods/assays used in Romania for diagnosis and surveillance of campylobacteriosis in animals.
• The experts coordinate pre-taking and drawing up the standards and diagnostic methods of campylobacteriosis in animals, in Romania.
• They carry out researches activities, assimilate and draw up the new analytical methods for laboratory diagnosis and surveillance of campylobacteriosis in animals.
• They participate together with the others national reference laboratories for campylobacteriosis in animals from Member States, to the international inter-comparison and proficiency tests organized by Community Reference Laboratory for Campylobacter.
• They participate to the quality assurance activities and method standardization for diagnosis, surveillance and screening of campylobacteriosis in animals, organized by Community Reference Laboratory for Campylobacter.
• The NRL specialists collaborate with Community Reference Laboratory for Campylobacter and benefit from training organized by this and supplying diagnostic standards, methods, reagents or diagnosis standards for campylobacteriosis in animals.
• The NRL experts collaborate with national reference laboratories for campylobacteriosis in animals from the others Member States, international reference laboratories of World Animal Health Organization (OIE) and other international accredited organizations in the field, in order to improve the surveillance and diagnostic capacity of campylobacteriosis in animals.
• In accordance with provisions of Diagnosis Manual, they carry out the laboratory tests/assays for diagnosis and surveillance of campylobacteriosis in animals and for national programmes for surveillance of zoonotic campylobacteriosis in animals.
• The NRL experts supply the diagnosis expertise for campylobacteriosis in animals in case of positive, suspect and inconclusive results obtained by the sanitary veterinary and food safety county laboratories, and of Bucharest, respectively.
• The NRL experts supply the diagnosis expertise for campylobacteriosis in animals, in case of dispute settlement.
• The NRL experts check and certify the diagnosis value of all diagnosis reagent batches and diagnosis sets or kits for campylobacteriosis in animals and designate their use by state sanitary veterinary laboratories from Romania.
• In preparation for yielding certain diagnosis reagents for campylobacteriosis in animals, supplying them for all authorised sanitary veterinary and food safety laboratories.
• They coordinate the drawing up of diagnosis standards and methods for campylobacteriosis in animals used by all authorized sanitary veterinary and food safety laboratories.
• The NRL experts perform the annual inter-laboratory comparative tests for checking the diagnostic capability for campylobacteriosis in animals, for all authorized sanitary veterinary and food safety county laboratories from county sanitary veterinary and food safety directorates.
• NRL also organize training and continuous training activities, in order to teach the specialists working in the authorized sanitary veterinary and food safety laboratories, on carrying out laboratory surveillance for campylobacteriosis in animals.
• The NRL experts carry out specific activities for identification and characterization the etiological agents of campylobacteriosis in animals, their origin and type.
• NRL has the responsibility to characterize the etiological agents of campylobacteriosis in animals, their genetic typing and designing the epidemiological genetic charts.
• They preserve the etiological agents of campylobacteriosis in animals under maximum safety conditions.
• The experts from NRL participate to the authorization of sanitary veterinary and food safety laboratories; they write down the checking files and assessment report and verify the achievement of quality insurance system conditions.
• Periodically, they communicate to the European Commission the list of authorized and accredited institutions or sanitary veterinary and food safety laboratories, having specific competences for campylobacteriosis in animals and also to notify any changes of the list.
• The NRL experts communicate, with the agreement of National Sanitary Veterinary and Food Safety Authority; the information requested by European Commission, Community Reference Laboratory for Campylobacter, World Animal Health Organization, and national reference laboratories for campylobacteriosis in animals from other Member States and other international accredited organizations and institutions in the field.
• The NRL experts provide, to National Sanitary Veterinary and Food Safety Authority and to sanitary veterinary and food safety county directorates, and of Bucharest, respectively, the official information on campylobacteriosis in animals with the aim of establishing the national strategies and policies on campylobacteriosis in animals.
• As a second or third part, they carry out advising activities, they grant technical advice and also they perform audit activities for institutions and organizations in animal husbandry or for those enterprises of which activities involve products or by-products derived from animals.
• In accordance with legislation in force, NRL promotes and implements the Good Laboratory Practice principles (GLP), to be used for applied research studies.
• They also participate to the drawing up, coordination, monitoring and carrying out some actions stipulated by “Programme for surveillance, prevention and control of animal diseases, those transmitted from animals to humans, animal and environmental protection”, yearly approved by National Sanitary Veterinary and Food Safety Authority President’s order.
• They participate to the establishing of maximum number of samples which are to be sampled for laboratory exams, regarding campylobacteriosis in animals, the maximum number of samples for laboratory exams done on pool samples and to calibration of national reference secondary sera against international primary standard sera.
• They participate to the elaboration of law drafts for campylobacteriosis in animals.
• They also participate to design the technical programs, the national and international projects regarding diagnosis and surveillance of campylobacteriosis in animals.
• The NRL experts participate or manage the research activities of national or international research programmes.

4. Activity in relation with correspondent Community Reference Laboratory

• Attendance to workshops
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• Attendance to trainings
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• Participation to the international inter–comparison and proficiency tests
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5. Activity in relation with correspondent national reference laboratories from Member States

• Attendance to workshops
  • –
• Attendance to trainings
  • –
• Participation to the international inter–comparison and proficiency tests
  • –

6. Activity in relation with correspondent international reference laboratory

• Attendance to workshops
  • –
• Attendance to trainings
  • –
• Participation to the international inter–comparison and proficiency tests
  • –

7. Activity in relation with other international accredited institutions in the field

• Attendance to workshops
  • –
• Attendance to trainings
  • –
• Participation to the international inter–comparison and proficiency tests
  • –

8. Activity in relation with counties SVFSLs

• Workshop organization
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• Training organization
  • 2008 – Isolation and identification methods for Campylobacter jejuni and Campylobacter coli species
  • 2009 - Isolation and identification techniques of Campylobacter jejuni and Campylobacter coli species
• Performing inter–comparison and proficiency tests
  • 2009 – Campylobacter spp. morpho-staining identification

9. Activity in relation with other institutions than SVFSLs or legal and private person

• Workshop organization
  • –
• Training organization
  • –
• Performing inter–comparison and proficiency tests
  • –