National Reference Laboratory for Contagious
Bovine Pleuropneumonia

1. NRL experts

• NRL coordinator

  Dr. Răgălie Adriana, PhD candidate
  tel. 0374.322.025
  e–mail: adriana.ragalie@idah.ro

• NRL deputy coordinator

  Dr. Rădulescu Ruxandra Alina, PhD in medical sciences, veterinary medicine speciality

• Team working

  Bg. Petriceanu Gela
  Dr. Guţu Emanuela, PhD candidate
  Dr. Ionescu Maria, PhD candidate
  Dr. Sandu Ion, PhD in medical sciences,veterinary medicine speciality
  Bg. Vanghele Luminiţa, PhD candidate
  Dr. Priocop Lavinia, PhD candidate
  Dr. Predescu Lucian
  Dr. Hiottu Anişoara, PhD in medical sciences, veterinary medicine speciality
  Dr. Pavel Nerone

2. Correspondent Community Reference Laboratory

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3. Specific atributions

• The specialists working in this NRL have the responsibility for drawing up the handbook „Manual for Contagious bovine pleuropneumonia Diagnosis”.
• They also perform the changes and/or supplements for „Manual for Contagious bovine pleuropneumonia Diagnosis” in accordance with new provisions of Community law and new recommendations of the World Animal Health Organization, established in „Manual of Diagnostic Tests and Vaccines for Terrestrial Animals” and „Terrestrial Animal Health Code”.
• NRL experts approve the results of validity studies proving the reliability of methods/assays used in Romania for diagnosis and surveillance of contagious bovine pleuropneumonia.
• The experts coordinate pre-taking and drawing up the standards and diagnostic methods of contagious bovine pleuropneumonia in Romania.
• They carry out researches activities, assimilate and draw up the new analytical methods for laboratory diagnosis and surveillance of contagious bovine pleuropneumonia.
• They participate together with the others national reference laboratories for contagious bovine pleuropneumonia from Member States, to the international inter-comparison and proficiency tests organized by international reference laboratories for contagious bovine pleuropneumonia and other international accredited institutions in the field.
• They participate to the quality assurance activities and method standardization for diagnosis, surveillance and screening of contagious bovine pleuropneumonia, organized by international reference laboratories for contagious bovine pleuropneumonia.
• The NRL specialists collaborate with international reference laboratories for contagious bovine pleuropneumonia and benefit from training organized by these and supplying diagnostic standards, methods, reagents or diagnosis standards for contagious bovine pleuropneumonia.
• The NRL experts collaborate with national reference laboratories for contagious bovine pleuropneumonia from the others Member States, international reference laboratories of World Animal Health Organization (OIE) and other international accredited organizations in the field, in order to improve the surveillance and diagnostic capacity of contagious bovine pleuropneumonia.
• In accordance with provisions of diagnosis manuals, they carry out the laboratory tests/assays for diagnosis and surveillance of contagious bovine pleuropneumonia.
• The NRL experts supply the diagnosis expertise for contagious bovine pleuropneumonia in case of positive, suspect and inconclusive results obtained by the sanitary veterinary and food safety county laboratories, and of Bucharest, respectively.
• The NRL experts supply the diagnosis expertise for contagious bovine pleuropneumonia in case of dispute settlement.
• The NRL experts check and certify the diagnosis value of all diagnosis reagent batches and diagnosis sets or kits for contagious bovine pleuropneumonia and designate their use by state sanitary veterinary laboratories from Romania.
• In preparation for yielding certain diagnosis reagents for contagious bovine pleuropneumonia, supplying them for all authorised sanitary veterinary and food safety laboratories.
• They coordinate the drawing up of diagnosis standards and methods for contagious bovine pleuropneumonia used by all authorized sanitary veterinary and food safety laboratories.
• The NRL experts perform the annual inter-laboratory comparative tests for checking the diagnostic capability for contagious bovine pleuropneumonia, for all authorized sanitary veterinary and food safety county laboratories from county sanitary veterinary and food safety directorates.
• NRL also organize training and continuous training activities, in order to teach the specialists working in the authorized sanitary veterinary and food safety laboratories, on carrying out laboratory surveillance for contagious bovine pleuropneumonia.
• The NRL experts proceed specific activities in order to identify and characterize the etiological agents of contagious bovine pleuropneumonia, their origin, type, subtype and variant.
• NRL has the responsibility to characterize the contagious bovine pleuropneumonia etiological agent strains, their genetic typing and designing the epidemiological genetic charts.
• They preserve the contagious bovine pleuropneumonia etiological agent strains under maximum safety conditions.
• The experts from NRL participate to the authorization of sanitary veterinary and food safety laboratories; they write down the checking files and assessment report and verify the achievement of quality insurance system conditions.
• Periodically, they communicate to the European Commission the list of authorized and accredited institutions or sanitary veterinary and food safety laboratories, having specific competences for contagious bovine pleuropneumonia and also to notify any changes of the list.
• The NRL experts communicate, with the agreement of National Sanitary Veterinary and Food Safety Authority, the information requested by European Commission, international reference laboratories, World Animal Health Organization, national reference laboratories for contagious bovine pleuropneumonia from other Member States and other international accredited organizations and institutions in the field.
• The NRL experts provide, to National Sanitary Veterinary and Food Safety Authority and to sanitary veterinary and food safety county directorates, and of Bucharest, respectively, the official information on contagious bovine pleuropneumonia with the aim of establishing the national strategies and policies contagious bovine pleuropneumonia.
• As a second or third part, they carry out advising activities, they grant technical advice and also they perform audit activities for institutions and organizations in animal husbandry or for those enterprises of which activities involve products or by-products derived from animals.
• Sometimes, the experts from NRL participate to inspection and control actions directed to contagious bovine pleuropneumonia, organized by National Sanitary Veterinary and Food Safety Authority.
• In accordance with legislation in force, NRL promotes and implements the Good Laboratory Practice principles (GLP), to be used for applied research studies.
• They also participate to the drawing up, coordination, monitoring and carrying out some actions stipulated by “Programme for surveillance, prevention and control of animal diseases, those transmitted from animals to humans, animal and environmental protection”, yearly approved by National Sanitary Veterinary and Food Safety Authority President’s order.
• They participate to the establishing of maximum number of samples which are to be sampled for laboratory exams, regarding contagious bovine pleuropneumonia and to calibration of national reference secondary sera against international primary standard sera.
• They participate to the elaboration of law drafts for contagious bovine pleuropneumonia.
• They also participate to design the technical programs, the national and international projects regarding contagious bovine pleuropneumonia diagnosis and surveillance.
• The NRL experts participate or manage the research activities of national or international research programmes.

4. Activity in relation with correspondent Community Reference Laboratory

• Attendance to workshops
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• Attendance to trainings
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• Participation to the international inter–comparison and proficiency tests
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5. Activity in relation with correspondent national reference laboratories from Member States

• Attendance to workshops
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• Attendance to trainings
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• Participation to the international inter–comparison and proficiency tests
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6. Activity in relation with correspondent international reference laboratory

• Attendance to workshops
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• Attendance to trainings
  • 1999 –“Laboratory diagnosis for Contagious bovine pleuropneumonia”, organized by International Reference Laboratory, IZS Teramo-Italy
  • 2003 -“Serological technics applied for Contagious bovine pleuropneumonia”, organized by International Reference Laboratory, Lisboa, Portugal
  • 2005 –“Laboratory diagnosis for Contagious bovine pleuropneumonia”, organized by International Reference Laboratory, Montpellier, France
• Participation to the international inter–comparison and proficiency tests
  • 2009 - Rig Trial on contagious bovine pleuropneumonia diagnosis, organized by International Reference Laboratory for Contagious Bovine Pleuropneumonia CIRAD – UMR15, Montpellier, France

7. Activity in relation with other international accredited institutions in the field

• Attendance to workshops
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• Attendance to trainings
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• Participation to the international inter–comparison and proficiency tests
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8. Activity in relation with counties SVFSLs

• Workshop organization
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• Training organization
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• Performing inter–comparison and proficiency tests
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9. Activity in relation with other institutions than SVFSLs or legal and private person

• Workshop organization
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• Training organization
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• Performing inter–comparison and proficiency tests
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